NAD+ 500mg

NAD+ 500 mg is a high-purity lyophilized nicotinamide adenine dinucleotide (NAD+) research compound supplied exclusively for scientific investigation and controlled laboratory experimentation.

For research use only — not for human or veterinary use.

Product Overview

Nicotinamide adenine dinucleotide (NAD+) is an essential biological coenzyme present in virtually every living cell and has become one of the most extensively studied molecules in modern cellular biology, mitochondrial research and biochemical science.

As a central component of numerous enzymatic reactions, NAD+ plays a critical role in cellular energy metabolism, oxidation-reduction reactions and intracellular biochemical communication. Because of its fundamental importance in cellular physiology, NAD+ continues to receive considerable attention in laboratory research investigating metabolic pathways, mitochondrial biology and molecular signalling.

This preparation contains 500 mg of high-purity lyophilized NAD+ supplied in a sealed research vial. Independent analytical testing confirmed an assay content of 550 mg for this production batch. Lyophilization preserves compound stability during storage while allowing controlled reconstitution for laboratory experimentation.

With its exceptionally high analytical purity, verified molecular identity and comprehensive third-party laboratory testing, NAD+ provides researchers with a highly characterised research material suitable for advanced biochemical and molecular investigations.

Compound Overview

NAD+ is a naturally occurring coenzyme utilised throughout biological systems and widely investigated in laboratory studies involving mitochondrial biology, cellular metabolism and enzymatic regulation.

Researchers employ NAD+ in controlled experimental models designed to investigate biochemical pathways, intracellular signalling, oxidation-reduction reactions and cellular energy production.

Its universal biological importance, highly conserved molecular structure and extensive scientific literature have established NAD+ as one of the most important compounds in biochemical and molecular research.

Today, NAD+ continues to appear throughout scientific literature involving mitochondrial function, metabolism, molecular biology, enzymology and cellular regulation.

Historical Background and Scientific Context

NAD+ has been investigated for more than a century and remains one of the most extensively studied biological molecules in modern science. Since its discovery in the early twentieth century, researchers have identified its involvement in hundreds of enzymatic reactions responsible for maintaining normal cellular function.

Advances in analytical chemistry, molecular biology and biochemical research have significantly expanded scientific understanding of NAD+, particularly regarding its participation in mitochondrial metabolism, intracellular signalling and cellular regulatory processes.

Modern analytical technologies including ultra-high-performance liquid chromatography, FTIR spectroscopy and high-resolution mass spectrometry now allow comprehensive verification of NAD+ identity, purity and manufacturing consistency for laboratory-grade research compounds.

Today, NAD+ remains a cornerstone of scientific investigation involving metabolism, mitochondrial biology, molecular signalling and biochemical regulation.

Mechanistic Focus in Research

Within controlled laboratory environments, NAD+ is commonly investigated in relation to cellular metabolism and biochemical signalling.

Research investigations frequently explore:

• Cellular energy metabolism
• Mitochondrial biology
• Oxidation-reduction reactions
• Intracellular signalling pathways
• Enzymatic regulation
• Molecular communication systems
• Biochemical pathway investigations

These experimental models allow researchers to investigate complex biochemical mechanisms governing cellular energy production and molecular regulation while maintaining carefully controlled laboratory conditions.

Analytical Verification (COA)

Each batch of NAD+ supplied by Grail Formula undergoes comprehensive independent third-party laboratory verification to confirm identity, assay content, purity and microbiological safety.

Analytical testing for this batch was performed by Liquilabs s.r.o. (Czechia) using validated chromatographic, spectrometric, microbiological and mass spectrometry techniques.

Batch: GF-NAD500-B242

• Assay Content: 550 mg
• Purity: greater than 99.8 percent
• Identification by FTIR Spectrum: 986
• Identification by Retention Time: 0.997
• Molecular Ion Mass Identification: 664 Da
• Bacterial Endotoxins: 0.009 EU/mg
• Total Aerobic Microbial Count: 0 CFU/g
• Total Yeast and Mold Count: 0 CFU/g

Independent elemental impurity analysis confirmed no detectable arsenic, cadmium, cobalt, lead, mercury, nickel or vanadium, with all measured concentrations remaining below the laboratory detection limit.

Additional microbiological testing confirmed no detectable aerobic microorganisms, yeasts or moulds, supporting laboratory-grade quality standards suitable for controlled scientific investigation.

These analytical procedures provide confirmation of compound identity, purity, traceability and batch consistency while ensuring every production batch meets Grail Formula’s strict research quality standards.

Research Applications

Within controlled laboratory environments, NAD+ may be utilised in experimental models designed to investigate mitochondrial biology, cellular metabolism, biochemical regulation and intracellular signalling pathways.

Examples of research applications include:

• Mitochondrial biology investigations
• Cellular metabolism research
• Biochemical pathway analysis
• Enzymatic regulation studies
• Intracellular signalling research
• Molecular biology investigations
• Controlled in vitro biochemical studies

These applications are intended exclusively for scientific and laboratory investigation.

Grail Formula Quality

Every Grail Formula research compound is manufactured under strict quality control procedures and supported by comprehensive independent analytical verification.

Each production batch undergoes extensive testing to verify:

• Compound identity confirmation
• Assay content verification
• Purity assessment
• Molecular ion mass verification
• Retention time confirmation
• FTIR spectrum identification
• Endotoxin analysis
• Microbial contamination screening
• Heavy metal screening
• Complete batch traceability

Independent laboratory testing helps ensure every batch provides reliable, reproducible research material suitable for advanced scientific investigation.

When You Choose Grail Formula

When selecting Grail Formula, researchers receive independently verified research compounds supported by complete Certificates of Analysis and transparent batch-specific laboratory documentation.

Our analytical verification programme includes assay determination, purity confirmation, FTIR spectrum identification, retention time verification, molecular ion mass identification, endotoxin screening, microbiological analysis and comprehensive heavy metal testing.

Every production batch is accompanied by a downloadable Certificate of Analysis issued by Liquilabs s.r.o. Czechia, providing complete traceability, independent verification and confidence in the analytical quality of every research compound supplied.

Research Use Limitation

• Used solely for laboratory and in vitro research
• Not approved for human consumption
• Not approved for veterinary use
• Not intended for diagnostic purposes
• Not intended for therapeutic applications
• Not permitted for clinical administration
• For research use only

• Independently analysed by Liquilabs s.r.o. Czechia
• Purity greater than 99.8 % confirmed by COA
• Assay content verified at 550 mg
• Identification confirmed by FTIR spectrum and retention time analysis
• Molecular ion mass identified at 664 Da
• Bacterial endotoxin level 0.009 EU/mg
• No detectable heavy metals or microbial contamination
• Research-grade NAD+ compound
• For research use only – not for human or veterinary use

For laboratory research only. Not intended for human consumption, injection, or cosmetic use.

This product is for research purposes only. Not for human use or diagnostic/therapeutic applications. Keep out of reach of children.